The toolkit is intended for use by a multisectoral team of country government leaders involved in the preparedness and response to public health events. The team should include senior policymakers and decision-makers from all relevant sectors, Preparedness and Response Management committees and their multisectoral members, and Rapid Response Team (RRT) leadership and members. Other participants can be included as needed, depending on each country’s One Health strategy, One Health platform structure, and government structure. This toolkit focuses on public health events that are not the result of intentional incidents (whether criminal or terrorism); in those cases, additional stakeholders, such as military or law enforcement, may be engaged in the response.